Abstract:This study adopted microwave pretreatment prior to enzymatic extraction of protein from Sinonovacula constricta. On the basis of single-factor trials，Plackett-Burman design was applied to screen four significant influencing factors，including microwave power，microwave treatment duration，enzymolysis temperature and enzymolysis duration. With the protein extraction rate of Sinonovacula constricta as the evaluation index，quadratic orthogonal rotation combination design was carried out to optimize the enzymatic extraction process，and the interaction effects between pairwise factors were further analyzed. The results demonstrated that under the fixed conditions of 1.4% compound enzyme dosage，solid-liquid ratio of 1∶5（g/mL） and enzymolysis pH of 7.5，the optimal technological parameters were microwave power of 600W，microwave duration of4.5 min，enzymolysis temperature of 53.0 ℃ and enzymolysis time of 180min. Under these optimized conditions，the protein extraction rate of Sinonovacula constricta protein reached （65.31±0.28）%.