Abstract:Barley grass meal replacement powder was prepared using barley grass，high-chain corn resistant starch，chia seeds，and steviol glycosides as raw materials. Single-factor experiments were conducted to investigate the effects of different raw materials on product quality，while orthogonal experiments were employed to optimize the optimal formulation of the barley grass meal replacement powder. Sensory evaluation methods were used to assess the product's quality. The results demonstrated that when the addition amounts were 3.0 g of barley grass powder，15 g of high-chain corn resistant starch，12 g of chia seeds，and 0.3 g of steviol glycosides，the product achieved a sensory score of 88，exhibiting a fresh aroma，smooth texture，and uniform color.Nutritional analysis revealed that the barley grass meal replacement powder contained 22.80%，dietary fiber of 43.35 g/100 g，and the product was rich levels of various vitamins and minerals，with calcium content 4.2 times higher than that of milk. Safety testing confirmed that the heavy metal levels complied with the requirements of GB 2762—2022 National Food Safety Standard Maximum Levels of Contaminants in Food，and the microbiological indicators met the requirements of GB 19640—2016 National Food Safety Standard Prepared Cereal Products.