Abstract:In this paper，by using purple sweet potato leaves as raw materials，anthocyanins were extracted by ultrasonic-assisted extraction technology. The antioxidant activity and biological activities related to the inhibition of HCC cell proliferation and metastasis of anthocyanins from purple sweet potato leaves were investigated，and the content of anthocyanins was determined by the pH differential method. The optimal extraction process was determined through single-factor experiments as follows：citric acid concentration of 3%，extraction time of 35 min，extraction temperature of 45 ℃，extraction power of 350 W，and material-to-liquid ratio of 1∶30 （g/mL）.The DPPH radical scavenging rates of both anthocyanins and vitamin C at a concentration of 500 μg/mL exceeded 90%. When the anthocyanin concentration was 140 μg/mL，the survival rate of HCC cells was only 40.2%，indicating that anthocyanins from purple sweet potato leaves could inhibit the survival of HCC cells.Scratch test results indicated that anthocyanins from purple sweet potato leaves had a significant inhibitory effect on the migration of HCC cells.