Abstract:To improve the utilization value of Asplenium nidus，this study adopted an ultrasonic-assisted method to prepare A. nidus powder. Taking protein content as the evaluation index，single-factor experiments were carried out to analyze the effects of ultrasonic frequency，power density，ultrasonic time and enzymatic hydrolysis conditions on the extraction efficiency of A. nidus. Meanwhile，the hypoglycemic，hypolipidemic and uric acid-lowering activities of the obtained powder were investigated in type 2 diabetic mice. The results showed that the optimal technological conditions were determined as follows：ultrasonic frequency of 20 kHz，power density of 1 950 W/L，ultrasonic time of 10 min，compound enzymolysis with cellulase and pectinase at the mass ratio of 1∶1，enzyme concentration of 20 U/mL，pH value of 4.5，enzymolysis temperature of 50 ℃，and enzymolysis time of 60 min. Under the optimized conditions，the protein content and soluble dietary fiber content of A. nidus powder were effectively increased. Compared with the model control group，the high dose of A. nidus powder （500 mg/kg） reduced the fasting blood glucose of diabetic mice by 49.1%. The levels of total cholesterol，triglyceride and low-density lipoprotein cholesterol were decreased by 55.8%，21.6% and 28.0%，respectively，and the serum uric acid level was reduced by 33.4%.