Abstract:To realize the efficient stepwise extraction of Dendrobium officinale polysaccharides and bibenzyl compounds，single-factor experiments combined with uniform design were employed in this study to optimize the extraction process of D.officinale polysaccharides. The residue obtained after polysaccharide extraction was directly reused for the extraction of bibenzyl compounds from D. officinale. On the basis of single-factor tests，response surface methodology was further applied to optimize the extraction technology of bibenzyl compounds.The results showed that the optimal conditions for polysaccharide extraction were enzymatic hydrolysis temperature of 65 ℃，enzymatic hydrolysis time of 120 min，solid-liquid ratio of 1∶45 （g/mL），and enzyme dosage of 1.5%. Under the verified conditions，the extraction rate of Dendrobium officinale polysaccharides reached 33.69%. The optimal conditions for the extraction of bibenzyl compounds were ultrasonic extraction time of 4 h，solid-liquid ratio of 1∶20 （g/mL），and ethanol volume fraction of 70%. Under verification experiments，the average extraction rate of bibenzyl compounds from Dendrobium officinale was 3.71%. The predicted values were in good agreement with the experimental measured values，which indicated that the experimental results were reliable and the optimized extraction process was feasible and effective.