Abstract:Enteritis is one of the major diseases leading to nutritional imbalances and health deterioration in the elderly population. Based on online databases，network pharmacology，and molecular docking techniques，this study investigated the potential co-regulatory mechanisms of threonine-tyrosine （TY），a peptide derived from the marine alga Porphyridium cruentum，in managing age-related enteritis and senescence. Relevant targets associated with enteritis，aging，and TY peptide were retrieved from online databases. A protein-protein interaction （PPI） network was constructed to screen core targets. Molecular docking was performed to validate the binding affinity between the core targets and TY peptide. Subsequently，co-expression and functional analyses of the core targets were conducted，and a transcription factor （TF）-miRNA-mRNA regulatory network was established. A Venn diagram analysis identified 54 overlapping targets. Based on topological parameters，14 core targets were further screened. Molecular docking and functional analyses of the top five core targets，which play key roles in inflammatory regulation，revealed that each docking model of TY peptide interacted with these five core targets and exhibited favorable binding affinities. Furthermore，analysis of the TF-miRNA-mRNA regulatory network predicted that hsa-miR-216a-3p and hsa-miR-6726-5p co-regulate the top five core targets.This study preliminarily elucidates the targets and mechanisms by which TY peptide may ameliorate enteritis and delay aging，providing a theoretical basis for the development of TY peptide-based functional foods tailored for the elderly population.