Abstract:On the basis of single-factor experiments，this study combined response surface optimization tests with polysaccharide yield as the evaluation index to screen the optimal process for ultrasound-assisted enzymatic extraction of chrysanthemum polysaccharides. High performance liquid chromatography （HPLC） combined with derivatization method was used to determine the monosaccharide composition and antioxidant capacity of chrysanthemum polysaccharides. The results showed that the optimal extraction conditions were extraction time of99 min，extraction temperature of52 ℃，solid-liquid ratio of 1∶36.5（g/mL） and enzyme dosage of 460 mg. Under these conditions，the yield of chrysanthemum polysaccharides reached （5.84±0.32）%.Monosaccharide composition analysis revealed that chrysanthemum polysaccharides consisted of mannose，glucuronic acid，glucose and fucose at a molar ratio of 1.00∶5.44∶1.55∶16.90. In-vitro antioxidant tests indicated that at the concentration of 5.0 mg/mL，the ABTS+radical scavenging rate of chrysanthemum polysaccharides was （51.12±6.38）%，and the DPPH radical scavenging rate was （56.39±2.76）%. The optimized ultrasound-assisted enzymatic extraction process was superior to hot-water extraction，single ultrasound extraction and single enzymatic extraction，which significantly improved the extraction yield of chrysanthemum polysaccharides. This study provides a reference for further development of chrysanthemum polysaccharides as natural antioxidants and functional foods.