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纳他霉素复合壳聚糖处理下采后黄皮货架期品质变化分析
李云昆1，2，3，李娅1，2*，郑晓灿1，2，3，陈岚1，2，杜丽清1，2*，谷会1，2，帅希祥1，2
(1.中国热带农业科学院南亚热带作物研究所/海南省热带园艺产品采后生理与保鲜重点实验室，广东湛江 524091；2.农业农村部热带果树生物学重点实验室，广东湛江 524091；3.云南农业大学热带作物学院，云南昆明 650500)
Postharvest Shelf Quality Changes in Wampee Fruits Treated with Natamycin Combined with Chitosan
LI Yunkun1，2，3，LI Ya1，2*，ZHENG Xiaocan1，2，3，CHEN Lan1，2，DU Liqing1，2*，GU Hui1，2，SHUAI Xixiang1，2
(1. South Subtropical Crops Research Institute，Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Postharvest Physiology and Technology of Tropical Horticultural Products of Hainan Province，Zhanjiang 524091，Guangdong，China；2. Key Laboratory of Tropical Fruit Biology，Ministry of Agriculture &Rural Affairs，Zhanjiang 524091，Guangdong，China；3. College of Tropical Crops，Yunnan Agricultural University，Kunming 650500，Yunnan，China)
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投稿时间：2025-04-11
中文摘要: 为研究纳他霉素复合壳聚糖处理对采后黄皮（25±1）℃货架期品质的影响，以“贵妃”黄皮为试验材料，以蒸馏水处理为对照组，采用不同浓度纳他霉素复合壳聚糖涂膜处理黄皮，比较其色泽、营养成分及其他生理生化指标的变化。结果表明，1 000 mg/L纳他霉素+1.0%壳聚糖处理组保鲜效果最佳，能有效延长采后黄皮货架期（贮藏8 d时，好果率达80％以上），延缓果实失重和褐变进程。与对照组相比，该复合保鲜剂处理有助于保持果实硬度和可溶性固形物含量，同时可减缓可滴定酸含量和维生素C（vitamin C，VC）含量的下降。进一步研究发现，该复合保鲜剂处理显著抑制黄皮果皮和果肉中丙二醛含量的上升，其含量分别比对照组低6.69 nmol/g和4.97 nmol/g（P＜0.05）。此外，复合保鲜剂处理还可以抑制多酚氧化酶的活性，使过氧化氢酶、过氧化物酶、超氧化物歧化酶的活性维持在较高水平。因此，适宜浓度的纳他霉素复合壳聚糖处理能有效抑制黄皮采后品质的下降，可作为一种绿色环保的方法应用于黄皮贮藏保鲜。
Abstract:To investigate the effects of natamycin combined with chitosan on the quality of postharvest wampee during shelf life at （25±1）℃，'Guifei' wampee [Clausena lansium （Lour.） Skeels] fruits were used as the experimental material，with distilled water treatment as the control. Wampee fruits were coated with different concentrations of natamycin combined with chitosan，and changes in color，nutritional components，and other physiological and biochemical indices were compared. The results revealed that the treatment with 1 000 mg/L natamycin+1.0% chitosan exhibited the best preservation effect，effectively extending the shelf life of postharvest wampee （the good fruit rate exceeded 80% after 8 d of storage） and delaying fruit weight loss and browning. Compared with the control group，the composite preservative treatment helped maintain fruit firmness and soluble solids content，while slowing the decline in titratable acidity and VC content. Further studies revealed that the composite preservative treatment significantly inhibited the increase in malondialdehyde content in both the peel and flesh of wampee，with values 6.69 nmol/g and 4.97 nmol/g lower than those of the control group，respectively （P＜0.05）. Furthermore，the composite preservative treatment inhibited polyphenol oxidase activity while maintaining higher activities of catalase，peroxidase，and superoxide dismutase. Therefore，treatment with an appropriate concentration of natamycin combined with chitosan can effectively inhibit the decline in postharvest quality of wampee and may serve as an environmentally friendly method for wampee storage and preservation.
文章编号：202615012 中图分类号： 文献标志码：
基金项目:以农产品为单元的广东省现代农业产业技术体系创新团队建设项目（2023KJ116）；海南省重点研发项目（ZDYF2023XDNY059）
| 作者 | 单位 |
| 李云昆 | 1.中国热带农业科学院南亚热带作物研究所/海南省热带园艺产品采后生理与保鲜重点实验室,广东湛江 524091；2.农业农村部热带果树生物学重点实验室,广东湛江 524091；3.云南农业大学热带作物学院,云南昆明 650500 |
| 李娅 | 1.中国热带农业科学院南亚热带作物研究所/海南省热带园艺产品采后生理与保鲜重点实验室,广东湛江 524091；2.农业农村部热带果树生物学重点实验室,广东湛江 524091； |
| 郑晓灿 | 1.中国热带农业科学院南亚热带作物研究所/海南省热带园艺产品采后生理与保鲜重点实验室,广东湛江 524091；2.农业农村部热带果树生物学重点实验室,广东湛江 524091；3.云南农业大学热带作物学院,云南昆明 650500 |
| 陈岚 | 1.中国热带农业科学院南亚热带作物研究所/海南省热带园艺产品采后生理与保鲜重点实验室,广东湛江 524091；2.农业农村部热带果树生物学重点实验室,广东湛江 524091； |
| 杜丽清 | 1.中国热带农业科学院南亚热带作物研究所/海南省热带园艺产品采后生理与保鲜重点实验室,广东湛江 524091；2.农业农村部热带果树生物学重点实验室,广东湛江 524091； |
| 谷会 | 1.中国热带农业科学院南亚热带作物研究所/海南省热带园艺产品采后生理与保鲜重点实验室,广东湛江 524091；2.农业农村部热带果树生物学重点实验室,广东湛江 524091； |
| 帅希祥 | 1.中国热带农业科学院南亚热带作物研究所/海南省热带园艺产品采后生理与保鲜重点实验室,广东湛江 524091；2.农业农村部热带果树生物学重点实验室,广东湛江 524091； |
| Author Name | Affiliation |
| LI Yunkun | 1. South Subtropical Crops Research Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Postharvest Physiology and Technology of Tropical Horticultural Products of Hainan Province,Zhanjiang 524091,Guangdong,China；2. Key Laboratory of Tropical Fruit Biology,Ministry of Agriculture &Rural Affairs,Zhanjiang 524091,Guangdong,China；3. College of Tropical Crops,Yunnan Agricultural University,Kunming 650500,Yunnan,China |
| LI Ya | 1. South Subtropical Crops Research Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Postharvest Physiology and Technology of Tropical Horticultural Products of Hainan Province,Zhanjiang 524091,Guangdong,China；2. Key Laboratory of Tropical Fruit Biology,Ministry of Agriculture &Rural Affairs,Zhanjiang 524091,Guangdong,China； |
| ZHENG Xiaocan | 1. South Subtropical Crops Research Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Postharvest Physiology and Technology of Tropical Horticultural Products of Hainan Province,Zhanjiang 524091,Guangdong,China；2. Key Laboratory of Tropical Fruit Biology,Ministry of Agriculture &Rural Affairs,Zhanjiang 524091,Guangdong,China；3. College of Tropical Crops,Yunnan Agricultural University,Kunming 650500,Yunnan,China |
| CHEN Lan | 1. South Subtropical Crops Research Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Postharvest Physiology and Technology of Tropical Horticultural Products of Hainan Province,Zhanjiang 524091,Guangdong,China；2. Key Laboratory of Tropical Fruit Biology,Ministry of Agriculture &Rural Affairs,Zhanjiang 524091,Guangdong,China； |
| DU Liqing | 1. South Subtropical Crops Research Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Postharvest Physiology and Technology of Tropical Horticultural Products of Hainan Province,Zhanjiang 524091,Guangdong,China；2. Key Laboratory of Tropical Fruit Biology,Ministry of Agriculture &Rural Affairs,Zhanjiang 524091,Guangdong,China； |
| GU Hui | 1. South Subtropical Crops Research Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Postharvest Physiology and Technology of Tropical Horticultural Products of Hainan Province,Zhanjiang 524091,Guangdong,China；2. Key Laboratory of Tropical Fruit Biology,Ministry of Agriculture &Rural Affairs,Zhanjiang 524091,Guangdong,China； |
| SHUAI Xixiang | 1. South Subtropical Crops Research Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Postharvest Physiology and Technology of Tropical Horticultural Products of Hainan Province,Zhanjiang 524091,Guangdong,China；2. Key Laboratory of Tropical Fruit Biology,Ministry of Agriculture &Rural Affairs,Zhanjiang 524091,Guangdong,China； |
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