Abstract:In this study，fermented lotus leaf polysaccharides （FJH-3） were extracted and isolated from lotus leaves fermented by Eurotium cristatum，and their regulatory effects on glucose metabolism in typeⅡdiabetic mice were investigated. The results showed that FJH-3 significantly reduced body weight，improved symptoms of polydipsia and polyphagia，regulated dyslipidemia，reduced the threat of cardiovascular disease to health，alleviated oxidative stress，and protected liver and pancreatic islet functions in mice. In addition，FJH-3 improved intestinal microecology，increased the abundance of beneficial bacteria （Bacteroidetes），and decreased the abundance of harmful bacteria （Lachnospiraceae）. These findings provide potential efficacy targets for the application of lotus leaf polysaccharides in the prevention and treatment of typeⅡdiabetes mellitus，demonstrating their potential in regulating blood glucose and improving intestinal health.