Abstract:To optimize the extraction process and evaluate the antioxidant activity of polysaccharides from Simao sweet bamboo shoots，the internal boiling method was employed. Single-factor experiments were conducted to optimize the extraction conditions，and the monosaccharide composition as well as antioxidant activity of the obtained polysaccharides were determined. The optimal conditions were as follows：resolution temperature 60 ℃，extraction time 6 min，resolution time 15 min，solid-to-liquid ratio 1∶30 （g/mL），resolving agent volume 6 mL，and resolving agent concentration 30%. Under these conditions，the average yield of polysaccharides was 7.5%. Monosaccharide composition and molecular weight analysis revealed that the polysaccharides were mainly composed of rhamnose，glucose，galactose，xylose，and mannose，with a weight-average molecular weight of 100 000 Da. Antioxidant activity assays showed that at a concentration of 5.0 mg/mL，the polysaccharides from Simao sweet bamboo shoots exhibited hydroxyl radical and superoxide anion radical scavenging activities equivalent to 52.18% and 81.33%，respectively，of that of 1.0 mg/mL L-ascorbic acid，indicating that the polysaccharides possess notable radical scavenging ability，with better efficacy against superoxide anion radicals. At 1.0 mg/mL，the crude polysaccharides showed DPPH radical scavenging activity equivalent to 50.58% of that of L-ascorbic acid at the same concentration. The total reducing power assay further confirmed that the polysaccharides from Simao sweet bamboo shoots have good reducing capacity.