Abstract:Taking Lycium barbarum crude polysaccharide content in the extract as the index，a single factor test was conducted to investigate the effects of ultrasonic time，temperature，and power on the crude polysaccharide content in the extract. Then，response surface experiment was performed to optimize the extraction conditions of Lycium barbarum crude polysaccharides. According to the results，the optimum ultrasonic extraction process was a time of 57.3 min，a temperature of 86.5 ℃，and a power of 164 W. Under these conditions，crude polysaccharide content in the extract was （0.631 6±0.002 6） mg/mL. Pigments and flavonoids in the extract were separated by adsorption and precipitation to obtain purified crude polysaccharide samples. At a concentration of 2.5 mg/mL，the DPPH free radical scavenging rate of the crude polysaccharide sample reached 80%.The cell experiment results showed that as the concentration of Lycium barbarum crude polysaccharides was increased，the survival rate of hepatocellular carcinoma （HCC） cells was gradually decreased. When the concentration of Lycium barbarum crude polysaccharides reached 140 μg/mL，the survival rate of HCC cells was only 44.4%，proving that the crude polysaccharides could inhibit HCC cell survival. Scratch experiments demonstrated that Lycium barbarum crude polysaccharide extract exhibited a good inhibitory effect on HCC cell metastasis.