Abstract:To investigate the optimal formulation of a high-dietary-fiber meal replacement powder made from Pyrus bretschneideri Rehd. and its in vitro lipid-lowering activity， a high-dietary-fiber meal replacement powder was prepared using Pyrus bretschneideri Rehd. pomace， konjac powder， xylitol， and aspartame as the main raw materials， with soy protein isolate as a filler. Using sensory score as the evaluation index， the formulation of the Pyrus bretschneideri Rehd. meal replacement powder was optimized through single-factor experiments and response surface methodology. The in vitro lipid-lowering activity of the Pyrus bretschneideri Rehd.meal replacement powder was analyzed via pancreatic lipase inhibition tests and cholesterol adsorption tests.The results showed that the optimal formulation for the Pyrus bretschneideri Rehd. meal replacement powder was based on a fixed total mass of 10 g， with soy protein isolate as the filler. The optimal amounts were： Pyrus bretschneideri Rehd. pomace powder 4.2 g， aspartame 20 mg， konjac powder 1.1 g， and xylitol 1.00 g. The meal replacement powder prepared under these conditions， when reconstituted， exhibited a pleasant sweet and sour taste， rich fruity aroma， and uniform texture， achieving the highest sensory score of 90.4. The soluble dietary fiber， insoluble dietary fiber， and total dietary fiber contents of the meal replacement powder were （8.36±0.25）%， （44.61±0.70）%， and （56.19±0.60）%， respectively. In vitro experimental results indicated that the maximum inhibition rate of pancreatic lipase by the Pyrus bretschneideri Rehd. meal replacement powder was（67.57±0.60）%. In a simulated small intestinal environment （pH7）， the maximum cholesterol adsorption rate was （64.138±0.667）%. In a simulated gastric environment （pH2）， the maximum cholesterol adsorption rate was （8.070±0.346）%. Therefore， the Pyrus bretschneideri Rehd. meal replacement powder exhibits good in vitro lipid-lowering activity.