Abstract:To investigate the effect of deep eutectic solvent （DES） extraction on the polysaccharides of Stropharia rugosoannulata， this study used Stropharia rugosoannulata as the raw materials and compared the differences in physicochemical properties of the polysaccharides obtained by DES extraction and traditional water extraction. The results showed that compared with water extraction， the extraction rate of Stropharia rugosoannulata polysaccharides by DES extraction was increased by 67.07%， with no significant difference in purity.Fourier transform infrared spectroscopy analysis indicated that the polysaccharides obtained by both extraction methods had typical characteristic absorption peaks of polysaccharides and were identified as acidic polysaccharides. Scanning electron microscope （SEM） observations showed that the surfaces of both polysaccharide samples were scaly， but significant differences were observed in their flake morphology and regularity. Monosaccharide composition analysis revealed that the polysaccharides from both extraction methods consisted of the same 10 monosaccharides. However， the relative content of uronic acid in the DES-extracted polysaccharides was higher. DSC analysis demonstrated that the endothermic enthalpy value of the DES-extracted polysaccharides was 16% higher than that of the water-extracted ones. Furthermore， both the emulsifying activity index （EAI） and emulsifying stability index （ESI） of the DES-extracted polysaccharides were higher than those of the water-extracted polysaccharides. Antioxidant activity evaluation showed that the antioxidant capacity of the Stropharia rugosoannulata polysaccharides extracted by DES was significantly superior to that extracted by water extraction.