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投稿时间:2025-05-20
投稿时间:2025-05-20
中文摘要: 为探究外源碳源菊糖对三角褐指藻岩藻黄素生物合成及碳代谢通路的调控作用,该试验以菊糖作为外源碳源,研究其对三角褐指藻岩藻黄素生物合成及碳代谢通路的调控作用。结果表明,添加菊糖显著提升了岩藻黄素的产量,其中,1 g/L处理组的岩藻黄素产量达到22.64 mg/L,较对照组显著提升39.67%(p<0.05)。叶绿素荧光参数测定发现,菊糖提升了三角褐指藻的电子传递效率和光合驱动力。基于非靶向代谢组学分析进一步揭示,菊糖通过增强呼吸代谢途径,加速了三羧酸(tricarboxylic acid,TCA)循环中关键中间产物的积累,进而促进了糖酵解(embdenmeyerhof-parnas,EMP)途径与TCA循环等中心碳代谢通路的活跃度,促进岩藻黄素积累,为功能食品的开发提供更充足的岩藻黄素原料。
Abstract:To investigate the regulatory effects of the exogenous carbon source inulin on fucoxanthin biosynthesis and carbon metabolic pathways in Phaeodactylum tricornutum(Pt.),this study employed inulin as an exogenous carbon source. The results revealed that inulin supplementation significantly enhanced fucoxanthin production,with the 1 g/L treatment group achieving 22.64 mg/L fucoxanthin yield,representing a 39.67% significant increase compared to the control group (p<0.05). Chlorophyll fluorescence parameter analysis demonstrated that inulin improved electron transport efficiency and photosynthetic driving force in Pt.. Untargeted metabolomics analysis further elucidated that inulin enhanced respiratlory metabolism by accelerating the accumulation of key intermediates in the tricarboxylic acid (TCA) cycle,thereby activating central carbon metabolic pathways including the embden-meyerhof-parnas (EMP) pathway and TCA cycle. These metabolic modifications collectively promoted fucoxanthin accumulation,providing enhanced raw material supplies for functional food development.
keywords: Phaeodactylum tricornutum fucoxanthin inulin mixotrophic cultivation physiological metabolism
文章编号:202612002 中图分类号: 文献标志码:
基金项目:国家自然科学基金项目(32400299);宁波市乡村振兴团队科技特派员项目(2024S217);宁波市科技计划项目(2024Z282);宁波市自然科学基金(2024J166);人才启动项目(705003G20230428)
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