食品研究与开发:2026,47(12):10-19
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菊糖对三角褐指藻细胞生理及光合碳代谢的影响
朱盈盈1,2,李越3,蔡路昀2,4,5 *,王晓娜6,李钰金7,徐冉2,4 *
(1.浙江万里学院 生物与环境学院,浙江 宁波 315100;2.浙江大学宁波国际科创中心,浙江 宁波315100;3.中国海洋大学 海德学院,山东 青岛 266100;4.浙江大学 生物系统工程与食品科学学院,浙江杭州 310058;5.宁波路鸣生物科技有限公司,浙江 宁波 315100;6.宁波宏纬食品有限公司,浙江 宁波315100;7.中国海洋大学 食品科学与工程学院,山东 青岛 266100)
Effects of Inulin on Physiology and Photosynthetic Carbon Metabolism of Phaeodactylum tricornutum
ZHU Yingying1,2,LI Yue3,CAI Luyun2,4,5 *,WANG Xiaona6,LI Yujin7,XU Ran2,4 *
(1. School of Biology and Environment,Zhejiang Wanli University,Ningbo 315100,Zhejiang,China;2. Ningbo International Innovation Center of Zhejiang University,Ningbo 315100,Zhejiang,China;3. Haide College,Ocean University of China,Qingdao 266100,Shandong,China;4. College of Biosystems Engineering and Food Science,Zhejiang University,Hangzhou 310058,Zhejiang,China;5. Ningbo Luming Biotechnology Co.,Ltd.,Ningbo 315100,Zhejiang,China;6. Ningbo Hongwei Foods Co.,Ltd.,Ningbo 315100,Zhejiang,China;7. College of Food Science and Engineering,Ocean University of China,Qingdao 266100,Shandong,China)
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投稿时间:2025-05-20    
中文摘要: 为探究外源碳源菊糖对三角褐指藻岩藻黄素生物合成及碳代谢通路的调控作用,该试验以菊糖作为外源碳源,研究其对三角褐指藻岩藻黄素生物合成及碳代谢通路的调控作用。结果表明,添加菊糖显著提升了岩藻黄素的产量,其中,1 g/L处理组的岩藻黄素产量达到22.64 mg/L,较对照组显著提升39.67%(p<0.05)。叶绿素荧光参数测定发现,菊糖提升了三角褐指藻的电子传递效率和光合驱动力。基于非靶向代谢组学分析进一步揭示,菊糖通过增强呼吸代谢途径,加速了三羧酸(tricarboxylic acid,TCA)循环中关键中间产物的积累,进而促进了糖酵解(embdenmeyerhof-parnas,EMP)途径与TCA循环等中心碳代谢通路的活跃度,促进岩藻黄素积累,为功能食品的开发提供更充足的岩藻黄素原料。
Abstract:To investigate the regulatory effects of the exogenous carbon source inulin on fucoxanthin biosynthesis and carbon metabolic pathways in Phaeodactylum tricornutum(Pt.),this study employed inulin as an exogenous carbon source. The results revealed that inulin supplementation significantly enhanced fucoxanthin production,with the 1 g/L treatment group achieving 22.64 mg/L fucoxanthin yield,representing a 39.67% significant increase compared to the control group (p<0.05). Chlorophyll fluorescence parameter analysis demonstrated that inulin improved electron transport efficiency and photosynthetic driving force in Pt.. Untargeted metabolomics analysis further elucidated that inulin enhanced respiratlory metabolism by accelerating the accumulation of key intermediates in the tricarboxylic acid (TCA) cycle,thereby activating central carbon metabolic pathways including the embden-meyerhof-parnas (EMP) pathway and TCA cycle. These metabolic modifications collectively promoted fucoxanthin accumulation,providing enhanced raw material supplies for functional food development.
文章编号:202612002     中图分类号:    文献标志码:
基金项目:国家自然科学基金项目(32400299);宁波市乡村振兴团队科技特派员项目(2024S217);宁波市科技计划项目(2024Z282);宁波市自然科学基金(2024J166);人才启动项目(705003G20230428)
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