Abstract:The effects of 1-naphthaleneacetic acid （NAA） on the metabolic pathways and bioactive substances of strawberry fruits were explored. The fruits of Fragaria ananassa Duch. cv. 'Akihime' were treated with 2 mmol/L NAA and distilled water （as control） and then stored at （20.0±0.5） ℃. Samples on day 8 of storage were used for non-targeted metabolomics analysis. The results showed that NAA treatment delayed the color change of strawberry fruits by increasing the L* and b* values while decreasing the a* value. Meanwhile，it enhanced the firmness and retarded the ripening process of the fruits. The non-targeted metabolomics analysis screened out 113 differential metabolites，of which 29 were up-regulated and 84 were down-regulated. A total of 22 compounds involved in the flavonoid biosynthetic pathway were identified. Kyoto Encyclopedia of Genes and Genomes（KEGG） enrichment analyses of differential metabolites revealed significant changes of metabolites involved in the biosynthetic pathways of flavonoids，flavone，and flavonol. In conclusion，NAA treatment regulated the color change and ripening process of strawberry fruits by modulating flavonoid metabolism，especially anthocyanin metabolism.