Abstract:To explore the repairing effect and underlying mechanism of Chinese yam-Astragalus-Polygonati Rhizoma compound nutritious soup on intestinal mucosal barrier injury in immunosuppressed model mice， an immunocompromised mouse model was established by intraperitoneal injection of cyclophosphamide （CTX）. Mice were divided into a normal group， a model group， a high-dose compound nutritious soup group （21.37 g/kg）， a medium-dose compound nutritious soup group （10.67 g/kg）， and a low-dose compound nutritious soup group（5.34 g/kg）. According to group assignment， mice were intragastrically administered equal volumes of normal saline or compound nutritious soup solution once daily for 7 days. Histopathological changes in intestinal tissues were observed by hematoxylin-eosin staining. Immunofluorescence staining was used to determine the positive localization of zonula occludens-1 （ZO-1） and occludin in the small intestine. Real-time quantitative polymerase chain reaction was performed to detect the mRNA expression levels of ZO-1， occludin， and polymeric immunoglobulin receptor （pIgR） in intestinal tissues. Western blot analysis was used to determine the protein expression levels of ZO-1， occludin， pIgR， and secretory immunoglobulin A （sIgA）. Enzyme-linked immunosorbent assay （ELISA） was used to measure the levels of cytokines interleukin-10 （IL-10） and interleukin-2 （IL-2）， the content of sIgA in intestinal tissues， and the content of lysozyme in intestinal mucosal mucus. The results showed that compound nutritious soup improved the general condition of CTX-induced immunocompromised mice， promoted body weight gain， increased the spleen index， and alleviated pathological damage in intestinal tissues. It also upregulated the expression of the tight junction molecules ZO-1 and occludin in the small intestinal epithelium， increased the levels of cytokines IL-10 and IL-2， and enhanced the activities of lysozyme， pIgR， and sIgA in mice， with the most pronounced effects observed in the high-dose compound nutritious soup group. In conclusion， compound nutritious soup can improve intestinal mucosal barrier injury in immunocompromised mice.