
码上扫一扫!
低糖山楂果丹皮的酶法制备、品质评价与体内代谢调节作用
孙铭璐1,2,姜敏1,2,王晓1,3,董红敬1,3,纪文华1,3,李涛1,3 *
(1.齐鲁工业大学(山东省科学院) 山东省分析测试中心 天然产物分离提取共性技术创新与应用山东省工程研究中心,山东 济南 250014;2.山东中医药大学 药学院,山东 济南 250355;3.齐鲁工业大学(山东省科学院) 药学院 山东省高等学校天然药物活性成分研究重点实验室,山东 济南 250014)
Enzymatic Preparation,Quality Evaluation,and in vivo Metabolic Regulation of Low-Sugar Hawthorn Sweetened Rolls
SUN Minglu1,2,JIANG Min1,2,WANG Xiao1,3,DONG Hongjing1,3,JI Wenhua1,3,LI Tao1,3 *
(1.Shandong Engineering Research Center for Innovation and Application of General Technology for Separation of Natural Products,Shandong Analysis and Test Center,Qilu University of Technology (Shandong Academy of Sciences),Jinan 250014,Shandong,China;2.School of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,Shandong,China;3.Key Laboratory for Natural Active Pharmaceutical Constituents Research in Universities of Shandong Province,School of Pharmaceutical Sciences,Qilu University of Technology (Shandong Academy of Sciences),Jinan 250014,Shandong,China)
本文已被:浏览 137次 下载 2次
投稿时间:2025-12-12
中文摘要: 采用葡萄糖氧化酶(glucose oxidase,GOD)、木糖醇和魔芋甘露寡糖制备低糖山楂果丹皮,通过感官评价、质构特性、色差、理化指标等分析其品质,并研究其对小鼠血糖和血脂的影响。结果表明:木糖醇、魔芋甘露寡糖或其复合物作为果丹皮蔗糖替代物制备的山楂果丹皮在表观形态、质构、色差及总糖含量方面与蔗糖果丹皮相比具有明显差异。与未添加0.3% GOD的魔芋甘露寡糖/木糖醇(25∶75)组相比,添加0.3% GOD制备的魔芋甘露寡糖/木糖醇(25∶75)复合果丹皮的弹性、胶黏性以及咀嚼性增加,而硬度和内聚性降低。GOD酶解后魔芋甘露寡糖、魔芋甘露寡糖/木糖醇(25∶75、50∶50和75∶25)制备果丹皮的葡萄糖含量与对应未酶解果丹皮相比分别显著降低了68.98%、64.71%、67.43%和61.57%(P<0.05);且无霉菌和大肠杆菌菌落存在。动物实验结果表明,GOD酶解魔芋甘露寡糖/木糖醇(25∶75)明显降低了小鼠的血糖和血脂水平,并提高其抗氧化能力。综上所述,添加0.3% GOD可以改善魔芋甘露寡糖和/或木糖醇制备山楂果丹皮的品质。
Abstract:Low-sugar hawthorn sweetened rolls were prepared using glucose oxidase (GOD),xylitol (Xyl),and konjac mannan oligosaccharide (KMOS).Then,their quality was analyzed by sensory evaluation,texture,color difference,and physicochemical indexes.Additionally,the effect of the rolls on blood sugar and lipid levels in mice was studied.The results showed that compared to the rolls formulated with sucrose,the rolls with Xyl,KMOS,or their complex as sucrose substitutes exhibited significantly different apparent morphology,texture,color difference,and total sugar content (P<0.05).Among the groups prepared with KMOS/Xyl (25∶75),the group supplemented with 0.3% GOD demonstrated increased elasticity,adhesiveness,and chewiness,as well as decreased hardness and cohesiveness.Compared with the corresponding unhydrolyzed rolls,the GOD-hydrolyzed rolls prepared with KMOS,KMOS/Xyl (25∶75,50∶50,and 75∶25) exhibited significantly decreased glucose content by 68.98%,64.71%,67.43%,and 61.57%,respectively (P<0.05),without molds or Escherichia coli.Animal experiments showed that the blood glucose and lipid levels in mice were significantly reduced,and the antioxidant capacity was enhanced in the KMOS/Xyl (25∶75) group after GOD enzymatic hydrolysis (P<0.05).In conclusion,the quality of the rolls prepared by KMOS and/or Xyl was improved with the addition of 0.3% GOD.
文章编号:202610003 中图分类号: 文献标志码:
基金项目:“十四五”国家重点研发计划项目(2023YFC3503805);泰山学者工程(tsqnz20231230);山东省自然科学基金青年项目(ZR2025QC274)
| 作者 | 单位 |
| 孙铭璐 | 1.齐鲁工业大学(山东省科学院) 山东省分析测试中心 天然产物分离提取共性技术创新与应用山东省工程研究中心,山东 济南 250014;2.山东中医药大学 药学院,山东 济南 250355; |
| 姜敏 | 1.齐鲁工业大学(山东省科学院) 山东省分析测试中心 天然产物分离提取共性技术创新与应用山东省工程研究中心,山东 济南 250014;2.山东中医药大学 药学院,山东 济南 250355; |
| 王晓 | 1.齐鲁工业大学(山东省科学院) 山东省分析测试中心 天然产物分离提取共性技术创新与应用山东省工程研究中心,山东 济南 250014;3.齐鲁工业大学(山东省科学院) 药学院 山东省高等学校天然药物活性成分研究重点实验室,山东 济南 250014 |
| 董红敬 | 1.齐鲁工业大学(山东省科学院) 山东省分析测试中心 天然产物分离提取共性技术创新与应用山东省工程研究中心,山东 济南 250014;3.齐鲁工业大学(山东省科学院) 药学院 山东省高等学校天然药物活性成分研究重点实验室,山东 济南 250014 |
| 纪文华 | 1.齐鲁工业大学(山东省科学院) 山东省分析测试中心 天然产物分离提取共性技术创新与应用山东省工程研究中心,山东 济南 250014;3.齐鲁工业大学(山东省科学院) 药学院 山东省高等学校天然药物活性成分研究重点实验室,山东 济南 250014 |
| 李涛 | 1.齐鲁工业大学(山东省科学院) 山东省分析测试中心 天然产物分离提取共性技术创新与应用山东省工程研究中心,山东 济南 250014;3.齐鲁工业大学(山东省科学院) 药学院 山东省高等学校天然药物活性成分研究重点实验室,山东 济南 250014 |
| Author Name | Affiliation |
| SUN Minglu | 1.Shandong Engineering Research Center for Innovation and Application of General Technology for Separation of Natural Products,Shandong Analysis and Test Center,Qilu University of Technology (Shandong Academy of Sciences),Jinan 250014,Shandong,China;2.School of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,Shandong,China;3.Key Laboratory for Natural Active Pharmaceutical Constituents Research in Universities of Shandong Province,School of Pharmaceutical Sciences,Qilu University of Technology (Shandong Academy of Sciences),Jinan 250014,Shandong,China |
| JIANG Min | 1.Shandong Engineering Research Center for Innovation and Application of General Technology for Separation of Natural Products,Shandong Analysis and Test Center,Qilu University of Technology (Shandong Academy of Sciences),Jinan 250014,Shandong,China;2.School of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,Shandong,China;3.Key Laboratory for Natural Active Pharmaceutical Constituents Research in Universities of Shandong Province,School of Pharmaceutical Sciences,Qilu University of Technology (Shandong Academy of Sciences),Jinan 250014,Shandong,China |
| WANG Xiao | 1.Shandong Engineering Research Center for Innovation and Application of General Technology for Separation of Natural Products,Shandong Analysis and Test Center,Qilu University of Technology (Shandong Academy of Sciences),Jinan 250014,Shandong,China;2.School of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,Shandong,China;3.Key Laboratory for Natural Active Pharmaceutical Constituents Research in Universities of Shandong Province,School of Pharmaceutical Sciences,Qilu University of Technology (Shandong Academy of Sciences),Jinan 250014,Shandong,China |
| DONG Hongjing | 1.Shandong Engineering Research Center for Innovation and Application of General Technology for Separation of Natural Products,Shandong Analysis and Test Center,Qilu University of Technology (Shandong Academy of Sciences),Jinan 250014,Shandong,China;2.School of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,Shandong,China;3.Key Laboratory for Natural Active Pharmaceutical Constituents Research in Universities of Shandong Province,School of Pharmaceutical Sciences,Qilu University of Technology (Shandong Academy of Sciences),Jinan 250014,Shandong,China |
| JI Wenhua | 1.Shandong Engineering Research Center for Innovation and Application of General Technology for Separation of Natural Products,Shandong Analysis and Test Center,Qilu University of Technology (Shandong Academy of Sciences),Jinan 250014,Shandong,China;2.School of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,Shandong,China;3.Key Laboratory for Natural Active Pharmaceutical Constituents Research in Universities of Shandong Province,School of Pharmaceutical Sciences,Qilu University of Technology (Shandong Academy of Sciences),Jinan 250014,Shandong,China |
| LI Tao | 1.Shandong Engineering Research Center for Innovation and Application of General Technology for Separation of Natural Products,Shandong Analysis and Test Center,Qilu University of Technology (Shandong Academy of Sciences),Jinan 250014,Shandong,China;2.School of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,Shandong,China;3.Key Laboratory for Natural Active Pharmaceutical Constituents Research in Universities of Shandong Province,School of Pharmaceutical Sciences,Qilu University of Technology (Shandong Academy of Sciences),Jinan 250014,Shandong,China |
引用文本: