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投稿时间:2024-09-05
投稿时间:2024-09-05
中文摘要: 为探究益生菌发酵草莓汁贮藏期的品质变化及其机制,以植物乳植杆菌LP121发酵草莓汁制备活菌型和灭菌型两种发酵饮料,将这两种饮料分别在4 ℃条件下贮藏30 d和37 ℃条件下贮藏60 d,探究这两种益生菌发酵草莓汁在贮藏期间的理化性质、抗氧化活性和香气成分的变化及规律。结果表明,将活菌型发酵草莓汁在4 ℃条件下贮藏30 d,低温降低了乳酸菌的代谢活动,贮藏结束后发酵草莓汁的乳酸菌菌落总数维持在107 CFU∕mL,总酸含量增加22.8%,总酚含量下降21.7%,总黄酮含量下降12.7%。其抗氧化活性有所波动,但与贮藏前无明显差异。灭菌型发酵草莓汁在37 ℃条件下贮藏60 d,高温促进了体系内的物质分解和活性物质的氧化反应,贮藏结束后其总酸含量增加27.17%,总酚含量下降8.6%,总黄酮含量下降15.7%。抗氧化活性与贮藏前相比也呈降低趋势,DPPH自由基清除率下降0.7%,羟自由基清除率下降2.9%。随贮藏时间的延长,对整体香气有益的醇类和酯类物质在活菌型发酵草莓汁中有所增加,而在灭菌型发酵草莓汁中呈现下降趋势。综上,在低温贮藏条件下,益生菌代谢活动能较好地保持活菌型益生菌发酵草莓汁的品质;在高温贮藏条件下,灭菌型益生菌发酵草莓汁由于体系内反应加快,品质有一定的下降。
Abstract:To investigate the quality changes and mechanism of probiotic-fermented strawberry juice during storage,Lactobacillus plantarum LP121 was used to prepare two kinds of fermented beverages: live and sterilized types.These beverages were stored at 4 ℃ for 30 d and 37 ℃ for 60 d,respectively,so as to analyze their changes in physicochemical properties,antioxidant activity,and aroma components during storage.The results showed that after storage at 4 ℃ for 30 d,the low temperature reduced the metabolic activity of lactic acid bacteria,and the colonies number of lactic acid bacteria colonies was maintained at 107 CFU∕mL.Total acid content increased by 22.8%,while total phenol and total flavonoid contents decreased by 21.7% and 12.7%,respectively.The antioxidant activity fluctuated but showed no significant difference compared with that before storage.For sterilized fermented strawberry juice stored at 37 ℃ for 60 d,high temperature promoted the decomposition of substances and oxidation of active compounds within the system.After storage,total acid content increased by 27.17%,while total phenol and total flavonoid contents decreased by 8.6% and 15.7%,respectively.Antioxidant activity also showed a declining trend compared to that before storage,with the DPPH free radical scavenging rate decreasing by 0.7% and the hydroxyl radical scavenging rate decreasing by 2.9%.With the prolongation of storage time,alcohols and esters beneficial to the overall aroma increased in the live fermented strawberry juice,while they showed a downward trend in the sterilized fermented strawberry juice.In conclusion,under low-temperature storage conditions,the metabolic activity of probiotics could effectively maintain the quality of live probiotic-fermented strawberry juice;under high-temperature storage conditions,the quality of sterilized probiotic-fermented strawberry juice declined due to accelerated reactions within the system.
文章编号:202609004 中图分类号: 文献标志码:
基金项目:济南市“新高校20条”资助项目(2021GXRC057)
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