本文已被:浏览 164次 下载 1次
投稿时间:2024-11-03
投稿时间:2024-11-03
中文摘要: 为探讨处理温度对猪血浆蛋白功能特性及结构的影响,以猪血浆蛋白为材料,采用不同温度(30~80 ℃)处理,分析检测蛋白溶解度、表面疏水性、浊度、起泡性和泡沫稳定性、乳化性和乳化稳定性、持水性和持油性、紫外吸收光谱、平均粒径、Zeta电位和自由巯基含量。结果表明,与30 ℃处理的猪血浆蛋白相比,蛋白溶解度、表面疏水性和浊度随着处理温度的升高整体呈上升趋势;起泡性、泡沫稳定性、乳化性和乳化稳定性随处理温度的升高波动变化;适当地进行高温处理,可使猪血浆蛋白的持水性减小而持油性增大,紫外吸收光谱的强度也增加;随处理温度的升高,蛋白平均粒径增大,而Zeta电位绝对值和自由巯基含量有降低趋势。综上,处理温度能有效改变猪血浆蛋白的功能与结构特性,为猪血浆蛋白功能开发及实际应用提供技术支持。
Abstract:To investigate the effects of treatment temperature on the functional properties and structural characteristics of porcine plasma protein,porcine plasma protein was treated at different temperatures (30-80 ℃).The solubility,surface hydrophobicity,turbidity,foaming capacity and foam stability,emulsifying capacity and emulsifying stability,water-holding capacity and oil-holding capacity,ultraviolet (UV) absorption spectrum,average particle size,Zeta potential,and free sulfhydryl content were determined and analyzed. The results showed that compared with porcine plasma protein treated at 30 ℃,the solubility,surface hydrophobicity,and turbidity of the protein exhibited an increasing trend with increasing treatment temperature. The foaming capacity and foam stability,as well as the emulsifying capacity and emulsifying stability,showed fluctuating changes with increasing treatment temperature. After treatment at appropriately high temperatures,the water-holding capacity of porcine plasma protein decreased,while its oil-holding capacity increased,and the intensity of the UV absorption spectrum increased. With increasing treatment temperature,the average particle size of porcine plasma protein increased,whereas the Zeta potential and free sulfhydryl content tended to decrease. In summary,treatment temperature can effectively alter the functional and structural characteristics of porcine plasma proteins,providing technical support for the development and practical application of porcine plasma protein functions.
keywords: porcine plasma protein treatment temperature solubility foaming capacity emulsifying capacity Zeta potential
文章编号:202607011 中图分类号: 文献标志码:
基金项目:河南省市场监督管理局科技计划项目(HNSCJGK202487);商丘市产品质量检验检测中心科研项目(2024001)
引用文本:

津公网安备12011602301139号