Abstract:To investigate the anti-fatigue effects of Lycium barbarum polysaccharides （LBP），zebrafish were used as a model organism. The experimental design included a normal control group，a model control group，a positive control group，and a double-dose control system experimental group constructed using L. barbarum aqueous extract and L. barbarum fruit （LBF）. The latter comprised an equivalent polysaccharide group treated with LBP-1 （aqueous extract of L. barbarum，870 μg/mL） and LBF （2 000 μg/mL），and a high-dose group treated with LBP-2 （aqueous extract of L. barbarum，2 000 μg/mL）. Except for the normal control group，the fatigue model in zebrafish was established using anhydrous sodium sulfite. After the corresponding treatments，biological indicators including lactic acid content，adenosine triphosphate （ATP） content，reactive oxygen species （ROS） concentration，and locomotor distance were measured in each group. The results showed that，compared with the model control group，lactic acid content and ROS concentration were significantly reduced，while ATP content and locomotor distance were significantly increased in the LBP-1，LBP-2，and LBF-treated groups （P<0.05）. Among these，LBP exhibited a more pronounced effect and played a dominant role.When used as a fatigue-ameliorating agent，the appropriate dose of LBP was 0-870 μg/mL. This study provides a multi-dimensional evaluation of the anti-fatigue effects of LBP and offers reference data for its application in the development of functional foods.