Abstract:This study compared the hypoglycaemic effects of fresh and dried D.officinale-R.glutinosa on type 2 diabetic mice.A high-fat and high-sugar diet combined with an intraperitoneal injection of streptozotocin was used to establish a mouse model of type 2 diabetes mellitus.The diabetic mice were randomly divided into a model group,a metformin-positive control group,high-and low-dose groups of fresh D.officinale-R.glutinosa,and high-and low-dose groups of dried D.officinale-R.glutinosa was set up simultaneously.During the gavage procedure,changes in body mass and fasting blood glucose were monitored.Following the gavage,serum biochemical indexes were measured,and pathological histological sections of the liver and pancreas were observed.The results showed that only the high-dose group of fresh D.officinale-R.glutinosa had a significant increase in body weight（P<0.05）,with a growth rate of 1.59%.Compared with the model group,the fasting blood glucose levels of mice in each D.officinale-R.glutinosa group were significantly lower from the 4th week of gavage（P<0.05,P<0.01）,and the high-dose group of fresh D.officinale-R.glutinosa had the most significant hypoglycaemic effect.The total cholesterol and triglyceride levels were significantly reduced in all the D.officinale-R.glutinosa group（P<0.05, P<0.01）.In the fresh D.officinale-R.glutinosa group,low-density lipoprotein cholesterol levels were significantly reduced（P<0.05, P<0.01）,while high-density lipoprotein cholesterol levels were significantly increased（P<0.05, P<0.01）.Insulin levels in the fresh D.officinale-R.glutinosa group were significantly reduced（P<0.01）.The insulin resistance index in each treatment group of D.officinale-R.glutinosa was significantly reduced（P<0.05）.Histopathological results showed that all treatment groups could repair the functional damage of liver and pancreatic cells in mice to varying degrees.In conclusion,D.officinale-R.glutinosa has been demonstrated to effectively reduce blood glucose and improve glucose-lipid metabolism and insulin resistance in type 2 diabetic mice,and the fresh D.officinale-R.glutinosa showed the most beneficial effect.