Abstract:This study aimed to investigate the protective effect of Panax notoginseng-glutathione compound powder（PG）on oxidative stress and vascular injury in aging rats.In the experiment,rats were randomly divided into a blank group,a model group,a vitamin E group,and PG groups at low,medium,and high doses.An aging model was established by D-galactose.Then,aspects below were observed in the rats：body weight,quality of life scores,organ indexes,carotid artery hematoxylin and eosin（HE）staining,plasma fibrinogen content and maximum platelet aggregation rate,serum oxidative stress factor,inflammatory factor,and vascular injury factor indicators,as well as transforming growth factor-β1（TGF-β1）/Smad pathway and relevant protein expression levels in renal tissues.Results showed that,compared with the model group,manifestations below were significant in PG groups at all doses：alleviated abnormal body weight increase and decreased quality of life scores,thinner carotid wall,wider vascular lumen,decreased fibrinogen（FIB）content and maximum platelet aggregation rate,increased total superoxide dismutase（T-SOD）and glutathione peroxidase（GSH-Px）activities,as well as 6-keto-prostaglandin F1α（6-keto-PGF1α）content,decreased malondialdehyde（MDA）,nitric oxide/endothelin-1（NO/ET-1）,thromboxane B2（TXB2）,interleukin-1β（IL-1β）,interleukin-6（IL-6）,and tumor necrosis factor-α（TNF-α）content,downregulated protein expression of transforming growth factor-β1（TGF-β1）,phosphorylated Smad2（p-Smad2）,phosphorylated Smad3（p-Smad3）,collagen type I（Collagen I）,and α-smooth muscle actin（α-SMA）,as well as upregulated vascular endothelial growth factor A（VEGF-A）expression.In conclusion,PG could exert a protective effect against vascular injury in D-galactose-induced aging rats via inhibiting the TGF-β1/Smad pathway to enhance antioxidant capacity and alleviate inflammatory response and vascular damage.