Abstract:To further elucidate the development and applications of selenium-enriched Hypsizygus marmoreus powder produced via solid-state fermentation，a comparative analysis was performed on the active components，including protein，flavonoids，and organic selenium，as well as the antioxidant activities of selenium-enriched powders prepared by two distinct processes. The results revealed that the solid-state fermented powder contained significantly elevated levels of soluble protein，total free amino acids，crude fat，flavonoids，organic selenium，and polysaccharides，compared to selenium-enriched fruiting body powder. At a specific concentration，the solid-state fermented powder also exhibited stronger superoxide anion scavenging ability and total reducing power. The DPPH free radical scavenging rates were comparable between the two powders，whereas the solidstate fermented powder was only lower in the hydroxyl radical scavenging ability.Considering the shorter cultivation cycle and reduced production costs，solid-state fermentation was more suitable as the primary strategy for the production of selenium-enriched Hypsizygus marmoreus powders.