Abstract:This study investigated bifidobacteria and lactobacilli isolated from human breast milk and infant feces as potential functional probiotics with inhibitory effects against foodborne drug-resistant bacteria，including resistant strains of Klebsiella pneumoniae，Staphylococcus aureus，Bacillus cereus，and Listeria monocytogenes.Using the Oxford cup method，we screened these isolates for antibacterial activity and further validated their efficacy in food substrates.The results showed that a total of 20 probiotic strains showing significant antibacterial activity against the target drug-resistant bacteria were obtained.Among them，four representative strains（Bifidobacterium longum FB1-1 and FB1-4，and Lactobacillus rhamnosus FL1-4 and FL1-11）demonstrated sustained antibacterial effects in the prefabricated sausage model，maintaining a removal rate exceeding 90% against all four drug-resistant pathogens during 6 hours of co-culture.