Abstract:To establish a quantitative detection and rapid screening method for foodborne poisoning caused by cortinarius.Cortinarius samples were extracted using an acetonitrile solution via ultrasonic extraction and purified through dispersive solid-phase extraction.Quantitative analysis and rapid screening of orellanine were performed based on ultra performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS） and multiple reaction monitoring-information dependent acquisition-enhanced product ion scan（MRM-IDA-EPI）.The results showed that through spiked recovery tests at low,medium,and high concentration levels,the spiked recovery rate of orellanine ranged from 91.7% to 107.7%,with a precision of 1.1%-2.5%.The linear correlation coefficient of orellanine was greater than 0.999 0 within the concentration range of 0.020-0.500 μg/mL.The method's detection limit and quantification limit were 10 μg/kg and 30 μg/kg,respectively,and the matrix effect coefficient K of the pretreatment method was 0.999.In summary,the established UPLC-MS/MS and MRMIDA-EPI determination methods for orellanine are accurate,with good reproducibility and high sensitivity,and they are suitable for quantitative analysis and rapid screening of foodborne poisoning caused by poisonous cortinarius.