Abstract:Ultra-high performance liquid chromatography tandem mass spectrometry （UHPLC-MS/MS） was used for non-targeted metabolomics analysis on ribeye stored at 4 ℃.The results indicated that a total of 914 metabolites were identified from the ribeye,mainly including lipids and lipid molecules,organic acids and derivatives,and organic heterocyclic compounds.Through the random forest method,40 potential differential metabolites were screened out.Cluster analysis and pathway enrichment analysis revealed that these differential metabolites primarily participated in nitrogen metabolism,glutathione metabolism,glyceric acid and dicarboxylic acid metabolism,arginine biosynthesis,pyrimidine metabolism,and pentose phosphate pathway.Lglutamic acid and L-glutamine were jointly involved in three different metabolic pathways,among which the metabolic pathways involving L-glutamic acid,glutathione,and γ-glutamylcysteine were of the greatest importance.