Abstract:Food safety has always been a focal issue for human health. Currently，more than 250 foodborne pathogens have been identified worldwide. It was not until recent years that some regions began to attach importance to the detection of foodborne pathogens and the causes of some foodborne diseases were gradually clarified. However，most conventional rapid food detection methods had technical limitations，which were not conducive to the detection of food samples. Three methods （isothermal amplification technology，microfluidic chips，and electrochemical sensors） for pathogen detection were reviewed，and the application prospects of different rapid detection technologies were discussed. Compared with conventional rapid detection technologies，these three methods had high efficiency，high sensitivity，and low requirements for professional literacy，being suitable for point-of-care testing. Meanwhile，these three methods had portability，which had a positive impact on the monitoring of food microorganisms at customs and can reduce costs. With the numerous import and export foods in China，the demand for pathogen screening is increasing day by day. These three methods are expected to occupy a mainstream position in future customs supervision.