Abstract:To establish an effective method for quality control and evaluation of black ginseng extract， this study utilized high performance liquid chromatography-tandem mass spectrometry （HPLC-MS/MS） to conduct an in-depth analysis of black ginseng extracts from different raw materials. The black ginseng extract samples were extracted with a 70% methanol solution. Separation was achieved using a Thermo Scientific-C18 column（100 mm×2.1 mm， 2.6 μm） with gradient elution. Quantitative analysis of 21 ginsenosides （ginsenosides Rg1， Re， Rf， Rb1， Rc， etc.） was performed using an electrospray ionization source in multiple reaction monitoring mode with an external standard method. An HPLC-MS/MS fingerprint was also constructed for identification and analysis. The research showed that the 21 ginsenosides exhibited good linear relationships within the concentration range of 0.2-10.0 μg/mL， with all correlation coefficients R2 greater than 0.998 6. The method demonstrated satisfactory repeatability， stability， precision， and recovery rate， meeting the requirements for quantitative analysis. The relative standard deviations （RSD） for the 21 ginsenosides ranged from 3.1% to 7.8%， and the recovery rate was between 88.8% and 99.8%. Among the tested black ginseng extract samples， one batch was found to contain pseudoginsenoside F11 and ginsenoside Rf， which were characteristic components of American ginseng， while ginsenoside Rf was detected in all other black ginseng extracts.