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投稿时间：2025-01-04
投稿时间：2025-01-04
中文摘要: 为优化双频超声波辅助提取潼川豆豉抗氧化肽的工艺条件，该研究在单因素试验的基础上，通过Plackett-Burman试验筛选得到pH值、料液比和超声功率密度3个影响显著因素，然后进行Box-Behnken试验，确定最佳工艺条件为pH7.9、料液比1∶37（g/mL）、超声功率密度297 W/L、超声时间30 min、25 kHz与40 kHz超声工作时间比30∶20（s/s）和超声温度40 ℃，在该工艺条件下，抗氧化肽提取率为（39.05±0.18）%、DPPH自由基清除率为（93.99±0.26）%，与对照相组比，分别提高了43.98%和22.34%。
Abstract:The response surface methodology was employed on the basis of single-factor test and the Plackett-Burman design to optimize dual-frequency ultrasound-assisted extraction of antioxidant peptides from Tongchuan Douchi in the present study. Three significant factors were screened according to the results of Plackett-Burman design，which were pH，material-to-liquid ratio，and ultrasound power density. The optimum process conditions were obtained by Box-Behnken design：pH 7.9，material-to-liquid ratio of 1∶37（g/mL），working time ratio of 25 kHz to 40 kHz to be 30∶20 （s/s），ultrasonic temperature of 40 ℃，ultrasound power density of 297 W/L，and ultrasonic time of 30 min. Under these conditions，the extraction rate of antioxidant peptides was（39.05±0.18）%，and the DPPH free radical scavenging rate was （93.99±0.26）%，which were 43.98% and 22.34% higher than those of the control，respectively.
keywords: Douchi antioxidant peptide ultrasound extraction antioxidant
DOI：10.12161/j.issn.1005-6521.2025.20.013
文章编号：202520013 中图分类号： 文献标志码：
基金项目:国家自然科学基金项目（32001642）；四川省科技厅重点研发项目（2024YFFK0430）；固态发酵资源利用四川省重点实验室开放基金项目（2018GTY007）
| Author Name | Affiliation |
| JIAN Xianyu | College of Life Science and Agri-forestry,Southwest University of Science and Technology,Mianyang 621000,Sichuan,China |
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