Abstract:This study uses oyster peptides as raw materials. Cell viability was detected using the cytotoxicity assay kit to confirm that oyster peptides had no toxic effect. An inflammation model of RAW264.7 cells was established，and the content of nitric oxide in RAW264.7 cells was determined. The secretion of the pro -inflammatory factor interleukin-1β（ IL-1β） was detected by enzyme-linked immunosorbent assay，and the messenger RNA（ mRNA） expression levels of cytokines，including monocyte chemotactic protein-1（MCP-1），interferon-β（ IFN-β），recombinant regulated on activation in normal T-cell expressed and secreted（ RANTES），interleukin-6（ IL-6），macrophage inflammatory protein-2（ MIP-2），and macrophage inflammatory protein-1α（MIP-1α） detected by real-time fluorescence quantitative polymerase chain reaction（ qPCR） to explore the effect of oyster peptides on related inflammatory factors. The expressions of inflammatory signaling pathway protein nuclear factor-k-gene binding p65 （NF-κB p65），recombinant myeloid differentiation factor 88（MYD88），NLR family，pyrin domain containing protein 3（ NLRP3），and ubiquitin binding protein 62（ p62）were detected by western blotting. The results showed that oyster peptides may exert anti-inflammatory effects by regulating the MYD88/NF-κB inflammatory signaling pathway and inflammasome NLRP3，thereby reducing the expressions of IL-1β，MCP-1，TGF-β，RANTES，IL-6，MIP-2，and MIP-1α .