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投稿时间：2024-11-11
投稿时间：2024-11-11
中文摘要: 为优化多花黄精多糖提取工艺，以多糖提取率为指标，通过单因素分析和响应面法，优化酶辅助提取多花黄精多糖工艺。结果表明，多花黄精多糖的最佳提取工艺条件为料液比1∶18（ g/mL）、复合酶添加量1.0%、纤维素酶与木瓜蛋白酶质量比3∶7、酶解温度53 ℃、酶解时间50 min，在此条件下，多糖提取率达20.89%。该提取液多糖浓度为50 mg/mL时，对DPPH自由基清除率为81.39%，IC50为21.12 mg/mL。利用该提取液制备的黄精艾尔精酿啤酒，符合国家相关标准并具有黄精特有香气和口感。
Abstract:To optimize the polysaccharide extraction from Polygonatum cyrtonema Hua，this paper aimed to optimize the enzyme-assisted extraction process using single-factor analysis and response surface methodology，with the polysaccharide extraction rate as the evaluation indicator. The results showed that the optimal extraction process conditions for polysaccharide were a solid-liquid ratio of 1∶18（g/mL），enzyme addition of 1.0%，a mass ratio of cellulase to papain of 3∶7，an enzymatic hydrolysis temperature of 53 ℃，and an enzymatic hydrolysis time of 50 min. Under these conditions，the extraction rate reached 20.89%. When the polysaccharide concentration of the extract was 50 mg/mL，the DPPH radical scavenging rate was 81.39%，with an IC50 value of 21.12 mg/mL. The Huangjing ale beer prepared using the aqueous extract of Polygonatum cyrtonema Hua meets national standards and possesses the unique aroma and flavor of Polygonatum.
keywords: Polygonatum cyrtonema Hua polysaccharide enzyme-assisted extraction process optimization beer
文章编号：202518014 中图分类号： 文献标志码：
基金项目:南平市资源化学产业科技创新联合资助项目（N2020Z007）；福建省科技厅高校产学合作项目（2021N5016）
| Author Name | Affiliation |
| LÜ Gan | 1. Fujian Provincial Key Laboratory of Eco-Industrial Green Technology,College of Ecology and Resources Engineering,Wuyi University,Wuyishan 354300,Fujian,China； |
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