Abstract:Type 2 diabetes mellitus （T2DM） is primarily caused by a combination of insulin resistance and insufficient function of pancreatic β-cells，which leads to an imbalance in the sugar metabolism. The mouse model of T2DM was administrated with chromium nicotinate and certain B vitamins by gavage for 28 consecutive days，and changes in body weight，food intake，blood glucose level，glucose tolerance，serum insulin，homeostatic model assessment for insulin resistance （HOMA-IR），homeostasis model assessment of β-cell function index （HOMA-β），and glucokinase （GK） were measured. The effects of these two substances on T2DM mice were assessed. The results showed that both chromium nicotinate and B vitamins，when used alone or in combination，at low，medium，and high doses，alleviated the weight loss and increased the food intake of T2DM mice to varying degrees. They significantly lowered the fasting blood glucose，serum insulin，and GK levels，reduced insulin resistance，and improved glucose tolerance.