Abstract:Based on the previously established QuEChERS-analyte protectant combined method， this study systematically investigated the effects of reconstitution solvent type， analyte protectant addition amount（concentration and volume）， pesticide concentration， and matrix concentration on the matrix effects of 36 pesticide residues. The results demonstrated that the choice of reconstitution solvent should be tailored to the specific characteristics of the target pesticides to effectively reduce matrix effects. The optimal protectant combination was 50 μL of a mixed protectant （3-ethoxy-1，2-propanediol at 10 mg/mL + L-gulonic acid γ-lactone at 1 mg/mL + D-sorbitol at 1 mg/mL）， which could enhance the response values of pesticides. For most pesticides， matrix effects were generally more pronounced at lower concentrations. When the pesticide concentration exceeded 0.05 μg/mL， the matrix effects for most pesticides were smaller. Generally， matrix effects could be attenuated by reducing the matrix concentration. However， for most pesticides， a 1-fold dilution of the matrix produced an insignificant reduction in matrix effects. In contrast， matrix effects decreased significantly when the dilution ratio reached 20-fold.