Abstract:To realize the high-value application of Ziziphus jujuba cv. Huizao resources from Xinjiang， the extraction process and activity of polysaccharides from Ziziphus jujuba cv. Huizao were studied. Firstly， the polysaccharide extraction rate was set as an index to compare four extraction methods： hot water， ultrasound-assisted method， microwave-assisted method， and deep eutectic system. Next， the response surface method was employed to optimize the conditions of the optimal extraction method. Then， the alcohol precipitation was conducted to obtain the crude polysaccharide， which was isolated and purified. Finally， the immune activities were compared before and after the isolation. The results showed that the hot water extraction method had the highest polysaccharide extraction rate of （3.93±0.25）%. The optimal process conditions were an extraction temperature of 82 ℃， an extraction time of 108 min， and a liquid-material ratio of 17∶1 （mL/g）. Under these conditions， the polysaccharide extraction rate was （4.18±0.53）%. The activity results showed that both crude polysaccharides and purified polysaccharides exhibited ideal activity， indicating that the optimized method improved the extraction rate while yielding polysaccharides with strong immune activity.