Abstract:In order to obtain rare ginsenoside probiotics with a high conversion rate，this study activated，separated，and cultivated the probiotic freeze-dried powder obtained from a self-separation bacterial strain library and then used the esculin plate colored method for preliminary screening，finally obtaining 18 probiotic strains that produced β-glucosidase.Arbutin was used for carbon source induction screening，and three strains with high β-glucosidase production were selected as probiotic strains. These three strains were then used for the fermentation of ginseng，resulting in a rare ginsenoside strain FTCM001 with a high conversion rate. After fermentation of this strain，the content of rare ginsenoside Rg3 increased by 0.020 mg/mL，representing a 66.7-fold increase compared to the pre-fermentation level. After morphological observation，16S rRNA gene sequence，pheS gene sequence，and physiological and biochemical characteristic analysis and identification，this strain was identified as Lactiplantibacillus plantarum.