Abstract:By taking the whole peanut plant as the raw material，the extraction method of trans-resveratrol from various parts of the plant was studied. The trans-resveratrol content was determined by high performance liquid chromatography（HPLC）and further confirmed by liquid chromatography-mass spectrometry（LC-MS）.The linear relationship，repeatability，precision，stability，and spiked recovery rate of the method were investigated.The results showed that when 80% methanol solution was used，and ultrasonic heating-assisted extraction was adopted，the trans-resveratrol in various parts of the peanut plants was completely extracted. When the peanut kernels were extracted，and the precipitating agent was used，the target substance could be well separated from other components. The determination of trans-resveratrol was not disturbed by impurity peaks，In the concentration range of 0.008 270-16.540 2 μg/mL for trans-resveratrol，the linear regression equation was Y=79 086.176 7X+47.050 3，with a correlation coefficient R2=1.000 0，and the linear relationship was good. The average recovery rate was 83.9%-104.0%，and the relative standard deviation was 0.20%-1.94%.The method was accurate and reliable，and it had good reproducibility，which was suitable for the determination of trans-resveratrol in peanut plants.