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投稿时间：2024-04-07
投稿时间：2024-04-07
中文摘要: 该研究采用组合效应系数（combination index，CI）方法评估咖啡因、米糠脂肪烷醇和葡萄糖联用抗疲劳的协同增效作用，并确定最优配方组成；通过大鼠负重游泳实验评估最佳配方的抗疲劳功效，同时应用比色法和酶联免疫吸附法测定血清、肝脏和肌肉组织内与疲劳及炎症相关指标；为探索其作用机制，采用实时荧光定量聚合酶链式反应（quantitative real-time polymerase chain reaction，qPCR）测定最佳配方对与疲劳相关的PIK3CG、Map2K2、Cdk2 和Rapgef3 基因相对表达量的影响。研究结果显示，在大鼠抗疲劳效应达到0.95 水平时，最佳配方为咖啡因2.2 mg/kg（占4.9%）、米糠脂肪烷醇14.3 mg/kg（占32.0%）和葡萄糖28.2 mg/kg（占63.1%），CI 值为0.1～0.3，显示三者配伍具有较强协同增效作用。大鼠抗疲劳功效实验显示，三者联用可以显著延长大鼠力竭游泳时间，明显降低血清中尿素氮（blood urea nitrogen，BUN）和乳酸（lactic acid，LD）的含量，能明显升高肝糖原（liver glycogen，LG）和肌糖原（muscle glycogen，MG）的水平。机制研究显示，三者联用能通过明显降低血清抗炎因子（白细胞介素IL-6、促炎因子IL-1β 和C-反应蛋白CRP）水平来改善疲劳；三者联用也能明显上调PIK3CG、Map2K2、Cdk2 和Rapgef3 基因表达，并且三者联用也明显优于咖啡因单独使用的抗疲劳功效。
Abstract:In this study，the three-component combination index （CI） method was used to assess the synergistic effect of caffeine，fatty alkanol from rice bran，and glucose in combination against fatigue and determine the optimal composition of the formula. The anti-fatigue efficacy of the best combination was assessed through a rat weight-loaded swimming experiment，along with the measurement of several fatigue-related indicators and inflammation-related indicators in serum，liver，and muscle tissue using calorimetry and enzyme linked immunosorbent assay. To explore the mechanism of action，quantitative real-time polymerase chain reaction （qPCR）was used to determine the effects of three-component compatibility on the relative expression of fatigue-related PIK3CG，Map2K2，Cdk2，and Rapgef3 genes. The calculation results showed that the optimal combination for achieving an anti-fatigue effect of 0.95 in rats was 2.2 mg/kg caffeine（4.9%），14.3 mg/kg fatty alkanol from rice bran （32.0%），and 28.2 mg/kg glucose （63.1%）. Under this combination，the CI value ranged from 0.1 to 0.3，indicating strong synergistic effects of three-component compatibility. The anti-fatigue efficacy test in rats showed that three-component compatibility significantly prolonged the swimming time after exhaustion，reduced the levels of blood urea nitrogen （BUN） and lactic acid （LD） in serum，significantly increased the levels of liver glycogen （LG） and muscle glycogen （MG）. Furthermore，three-component compatibility significantly improved fatigue by reducing the levels of serum anti-inflammatory factors （interleukin IL-6，proinflammatory factor IL-1β，and C-reactive protein CRP） and could significantly upregulate the expression of the PIK3CG，Map2K2，Cdk2，and Rapgef3 genes. Additionally，the effect was significantly superior to the antifatigue effect of caffeine alone. The above results showed that the three-component CI method can determine the optimal composition of components based on efficacy experiments and evaluate the synergistic effects between components，providing a scientific method for the combination use of multiple components.
keywords: combination index （CI） synergistic effect caffeine fatty alkanol from rice bran anti-fatigue
文章编号：202514016 中图分类号： 文献标志码：
基金项目:中国中医科学院科技创新工程项目（CI2021A5203）
| 作者 | 单位 |
| 赵建1，崔洪润2，于静涛2，王云3，田纪祥3* | 1. 北京联合大学 生物化学工程学院,北京 100023；2. 通化一洋保健品有限公司,吉林 通化 134100；3. 中国中医科学院 中药研究所 道地药材品质保障与资源持续利用全国重点实验室,北京 100700 |
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