Abstract:In this article，QuEChERS（quick，easy，cheap，effective，rugged and safe）-liquid chromatographyhigh resolution mass spectrometry（LC-HRMS）was employed to establish a rapid detection method for 34 pesticides in fruits and vegetables，and to build a database.The sample extraction condition was 4 g of anhydrous magnesium sulfate，1.5 g of anhydrous sodium acetate，1 g of sodium chloride，and 10 mL of acetonitrile（1%acetic acid）. The purification agent condition was 10 mg of graphitized carbon black，500 mg of ethylenediamine-N-propyl silanized silica gel，600 mg of MgSO4，and 200 mg of C18.Under the acetonitrile-water mobile phase condition，the C18 chromatography column was selected for target separation.The samples were detected in Full MS/dd-MS2 mode，with results quantified by retention time，first-order exact molecular weight and second-order fragments，and qualified by first-order exact molecular weight. In the quantification，the matrixmatched standard curve was used，with chives，spinach，eggplants，grapes，peaches，and bananas as representative samples. It was shown in the result that each pesticide showed a good linear relationship within the corresponding linear range，with R2 greater than 0.99，a detection limit of 1-5 μg/kg，a quantification limit of 2-10 μg/kg，a recovery rate range of 70.06%-119.79% and a precision of 0.23%-13.77%. This method is simple，fast，super accurate，and highly sensitive，suitable for detecting 34 pesticides in vegetables and fruits.