Abstract:This research focused on optimizing the production parameters for generating angiotensin-converting enzyme suppressing oligopeptide from defatted wolfberry seed meal through enzymatic hydrolysis.Initial investigations examined the individual effects of critical processing variables - including protease concentration，sonication intensity，digestion duration，reaction temperature，and substrate ratio - on proteolytic efficiency and antihypertensive activity，as measured by degree of hydrolysis and ACE inhibition capacity.Building upon these preliminary findings，response surface methodology was employed to identify optimal interactive conditions among these parameters，with particular emphasis on maximizing ACE-inhibitory potential. The optimized protocol yielded peptides exhibiting superior ACE inhibition（71.28±0.86）% when prepared under the following conditions：alkaline protease dosage of 1.42×10?U/g，123 W ultrasonic treatment，36.5 min enzymatic digestion at 50 ℃.