Abstract:In this study，rapeseed was utilized as the raw material for oil extraction and rapeseed meal collection using four methods of screw pressing，hydraulic pressing，solvent extraction，and aqueous enzymatic extraction to compared the effects of these extraction methods on the quality of rapeseed oil and rapeseed protein.The results indicated significant differences in the physicochemical properties of the rapeseed oil extracted by the four methods. The acid value was highest in the screw-pressed oil at 0.58 mg/g，while the saponification value was highest in the hydraulically pressed oil at 189.91 mg/g. The fatty acid composition and content showed little variation，with five fatty acids detected in all samples，among which oleic acid content was the highest and stearic acid the lowest. The solvent-extracted oil exhibited the smallest change in peroxide value，indicating the highest oxidative stability among the four samples，whereas the aqueous enzymatic extraction method resulted in the least stable oil.Also，the extraction methods differentially affected the infrared spectral peak intensities，and the contents of β-helix and β-folding in the rapeseed protein. The hydraulically pressed protein showed the highest water-holding and oil-holding capacities，while the protein extracted with the aqueous enzymatic method was lowest in foaming，water-holding，and oil-holding capacities. The screw-pressed protein demonstrated the lowest solubility.