食品研究与开发:2025,46(10):148-154
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纤维素酶/木聚糖酶复合酶解竹笋粉的工艺优化及其功能特性
(1.安徽省农业科学院 农产品加工研究所,安徽 合肥 230001;2.安徽省食品微生物发酵与功能应用工程实验室,安徽 合肥 230001;3.安徽科技学院 食品工程学院,安徽 滁州 233100)
Process Optimization and Functional Characterization of Bamboo Shoot Powder Modified by Composite Cellulase/Xylanase Hydrolysis
(1. Institute of Agro-products Processing,Anhui Academy of Agricultural Sciences,Hefei 230001,Anhui,China;2. Anhui Engineering Laboratory of Food Microbial Fermentation and Functional Application,Hefei 230001,Anhui,China;3. College of Food Engineering,Anhui Science and Technology University,Chuzhou 233100,Anhui,China)
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投稿时间:2023-11-09    
中文摘要: 以可溶性膳食纤维含量为指标,优化纤维素酶和木聚糖酶复合酶解竹笋粉的工艺条件。结果表明,在料液比为1∶10 (g/mL),复合酶添加量为0.4%时,最佳酶解条件为酶解温度50 ℃、pH4.6、酶解时间90 min、纤维素酶与木聚糖酶质量比2∶1,在该条件下得到可溶性膳食纤维得率为13.94%。扫描电子显微镜和X 射线衍射结果表明,酶解后竹笋的结构变得更疏松多孔,结晶度下降,持水力、膨胀力、持油力和DPPH 自由基清除率均明显提高,说明复合酶解可显著改善竹笋膳食纤维的功能特性。
Abstract:The composite enzymatic (cellulase and xylanase) hydrolysis conditions of bamboo shoot powder were optimized based on the soluble dietary fiber content. The enzymatic hydrolysis conditions were optimized as follows:a solid-to-liquid ratio of 1∶10 (g/mL),a composite enzyme addition amount of 0.4% (a cellulase-toxylanase mass ratio of 2∶1),and enzymatic hydrolysis at 50 ℃ and pH4.6 for 90 min. Under these conditions,the yield of soluble dietary fiber reached 13.94%. Scanning electron microscopy and X-ray diffraction results revealed that the enzymatic hydrolysis resulted in a more loose and porous structure of bamboo shoots,accompanied by a decrease in crystallinity. Furthermore,the functional properties of bamboo shoot dietary fiber were significantly improved,as evidenced by the enhanced water-holding capacity,expansion capacity,oil-holding capacity,and DPPH free radical scavenging ability. These findings demonstrated that composite enzymatic hydrolysis effectively enhanced the functional properties of bamboo shoot dietary fiber.
文章编号:202510019     中图分类号:    文献标志码:
基金项目:淮北市科技计划项目 (HK2022AB008);安徽省科技重大专项(202003a06020007/02)
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