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投稿时间：2023-11-14
投稿时间：2023-11-14
中文摘要: 为研究莴苣叶中不溶性膳食纤维提取的最佳工艺条件，以莴苣叶渣为原料，采用碱提法提取不溶性膳食纤维（insoluble dietary fiber，IDF）。选取NaOH 浓度、液料比、提取时间和提取温度为因素，以IDF 得率为指标，通过响应面法对提取工艺进行优化，并对样品进行表征及应用性能评价。结果表明，最优提取工艺条件为NaOH 浓度5.5%、液料比40∶1（mL/g）、提取时间15 min、提取温度65 ℃，此时IDF 得率为51.43%。通过傅里叶变换红外光谱仪分析可知提取的IDF 是典型的膳食纤维，由纤维素、半纤维素、木质素和果胶4 种主要成分组成，通过X 射线衍射分析可知提取的IDF 属于纤维素Ⅰ型晶体结构，通过热重分析可知提取的IDF 具有更高的热解温度和更好的热稳定性。IDF 的持水力和持油力均较高，分别为（5.80±0.16）g/g 和（1.76±0.17) g/g。
Abstract:Insoluble dietary fiber（IDF）was obtained from lettuce leaf residue by alkali extraction.The extraction conditions including NaOH concentration，liquid-to-solid ratio，extraction time，and extraction temperature were optimized by response surface methodology with IDF yield as the indicator.The obtained IDF was characterized and its application performance was evaluated.The results showed that the optimal extraction process was extraction with 5.5% NaOH and a liquid-to-solid ratio of 40∶1（mL/g）at 65 ℃for 15 min，under which the IDF yield was 51.43%.The extracted IDF was a typical dietary fiber composed of four main components：cellulose，hemicellulose，lignin，and pectin，according to the results of Fourier transform infrared spectrometry.The X-ray diffraction results indicated that the extracted IDF presented a type I crystal structure of cellulose.The thermogravimetry results suggested that the extracted IDF had higher pyrolysis temperature and better thermal stability.In addition，the extracted IDF showcased the water and oil holding capacity of（5.80±0.16）g/g and（1.76±0.17)g/g，respectively.
keywords: lettuce leaf residue insoluble dietary fiber alkali extraction extraction process optimization
文章编号：202411021 中图分类号： 文献标志码：
基金项目:大学生创新创业训练计划项目（S202310240133）
| Author Name | Affiliation |
| WU Xiaohan，LUO Yueteng，LIU Meng，DONG Yuqi，LIU Yuqi，CHE Chunbo* | School of Food Science and Engineering，Harbin University of Commerce，Harbin 150028，Heilongjiang，China |
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