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投稿时间：2023-12-02
投稿时间：2023-12-02
中文摘要: 为提高竹虫利用率，以竹虫为原料，考察不同酶、pH 值、提取温度、提取时间、液料比、加酶量对竹虫蛋白提取率的影响，通过响应面试验优化提取工艺，并测定竹虫蛋白的功能特性。结果表明：采用胃蛋白酶酶解，pH2.0、提取温度40 ℃、提取时间2.0 h、液料比12.5∶1（mL/g）、加酶量1.0%时提取工艺最佳，此时竹虫蛋白提取率为21.00%。竹虫蛋白对DPPH?、ABTS+?清除率的IC50分别为0.43 mg/mL 和0.66 mg/mL，其起泡性、乳化性和乳化稳定性良好。
Abstract:Protein was extracted from Omphisa fuscidentalis to improve the utilization of this insect.The effects of different enzymes，pH，extraction temperature，extraction time，liquid-to-solid ratio，and enzyme dosage on the extraction rate of O.fuscidentalis protein were studied.The extraction process was optimized by response surface methodology and the properties of O.fuscidentalis protein were determined.The results showed that the optimal extraction conditions were extraction with the liquid-to-solid ratio of 12.5∶1（mL/g）and 1.0% pepsin at pH2.0 and 40 ℃for 2.0 h.The extraction rate of O.fuscidentalis protein was 21.00%.The half maximal inhibitory concentrations（IC50）of O.fuscidentalis protein for scavenging DPPH· and ABTS+·were 0.43 mg/mL and 0.66 mg/mL，respectively.The extracted O.fuscidentalis protein demonstrated prominent foamability，emulsibility，and emulsifying stability.
keywords: enzymatic extraction Omphisa fuscidentalis protein extraction rate antioxidant activity properties
文章编号：202411020 中图分类号： 文献标志码：
基金项目:2022年云南省大学生创新创业训练计划项目（S20221067006）；云南省“兴滇英才支持计划”青年人才专项资助（YNWR-QNBJ-2018-046）
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