Abstract:This study established an detection method for the rapid determination of residues of six common herbicides in animal-derived foods using QuEChERS-ultra high-performance liquid chromatography-tandem mass spectrometry.Samples such as meat and offal of livestock and poultry，eggs，and raw milk were extracted with a 10% formic acid acetonitrile solution by volume.The extracts were purified with C18 powder and anhydrous magnesium sulfate，separated using a Hypersil GOLDTM C18 chromatographic column，and eluted with a gradient of methanol and 0.1% formic acid aqueous solution as the mobile phase.Quantification was performed using the matrix-matched standard curve external standard method.The results showed that the linear relationships of saflufenacil，bentazone，cycloxydim，and pinoxaden were good in the range of 0.2 μg/L to 20.0 μg/L，and aminopyralid and aminocyclopyrachlor in the range of 1.0 μg/L to 50.0 μg/L，with correlation coefficients（R2）all greater than 0.995.The limit of quantification（LOQ）of the method was 1.0 μg/kg.For beef，chicken eggs，pork liver，and cow milk as matrices，the recovery rates of the six herbicides at three spiked levels（1.0，5.0 μg/kg，and 20.0 μg/kg）were 72.8% to 98.5%，with relative standard deviations of 1.13% to 6.28%.