Abstract:Lactic acid bacteria were isolated from the natural fermentation broth of sticky bean bun.The strains were identified and their probiotic properties were studied.Gradient dilution method was used to isolate 6 strains of lactic acid bacteria from sticky bean bun.Morphological and 16S rRNA sequence analysis showed that they were Lactobacillus coryniformis，Lactobacillus plantarum，Levilactobacillus brevis，Lactobacillus fermentans，Latilactobacillus sakei，and Lactobacillus curvatus.In probiotic effect evaluation，strain HSDF3（L.curvatus）showed good stress and acid resistance，and the survival rate was 100% at pH3.0.In salt tolerance test，strain HSDF9（L.sakei）had the best salt tolerance，survived in MRS broth with 10% salt content，and the viable count could reach 8.93×106 CFU/mL.In bile salt tolerance test，strain HSDF12（L.coryniformis）had the highest survival rate，which was 7.171% after 3 h of incubation in MRS broth with 0.3% bile salt.In test of resistance to artificial gastric juice，after 3 h of incubation in simulated gastric juice，the survival rate of strain HSDF3（L.curvatus）was the highest，reaching 82.91%.In test of resistance to artificial intestinal fluid，after 6 h of incubation simulated intestinal fluid，the survival rate of HSDF12（L.coryniformis）was the highest，reaching 93.42%.In antibiotic sensitivity test，strain HSDF15（L.brevis）was sensitive to tetracycline，carboxybenzillin，cephalexin，minocycline，cefazolin，ampicillin，and cefradine.