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投稿时间：2023-01-30
投稿时间：2023-01-30
中文摘要: 为探究黄线狭鳕鱼皮胶原蛋白肽对D-半乳糖致衰老小鼠改善作用，通过建造D-半乳糖致衰老小鼠模型，以制备的黄线狭鳕鱼皮胶原蛋白肽为研究对象，选用低剂量（200 mg/kg）、中剂量（400 mg/kg）和高剂量（800 mg/kg）胶原蛋白肽灌胃D-半乳糖致衰老小鼠模型，对皮肤抗衰老作用进行研究。通过测定小鼠皮肤中的水分、胶原蛋白、羟脯氨酸（hydroxyproline，HYP）、透明质酸（hyaluronic acid，HA）含量、小鼠血清和皮肤中超氧化物歧化酶（superoxide dismutase，SOD）、过氧化氢酶（catalase，CAT）活性、丙二醛（malondialdehyde，MDA）含量和谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-Px）活性以及对小鼠皮肤组织形态学的观察，评价黄线狭鳕鱼皮胶原蛋白肽抗衰老作用效果。结果表明，黄线狭鳕鱼皮胶原蛋白肽可以显著提高D-半乳糖致衰老小鼠皮肤的水分、胶原蛋白、HYP 和HA 的含量，其中高剂量胶原蛋白肽组的效果最显著。此外，黄线狭鳕鱼皮胶原蛋白肽能显著增加衰老小鼠血清和皮肤中SOD、CAT 活性、提升GSH-Px 活性，并有效抑制MDA 的产生。通过病理切片观察小鼠皮肤组织形态，发现黄线狭鳕鱼胶原蛋白肽能有效减轻因衰老引起的皮肤粗糙、松弛、胶原纤维减少、排列障碍等现象。综上，黄线狭鳕鱼皮胶原蛋白肽对D-半乳糖致衰老小鼠有明显改善作用，有效延缓皮肤的老化进程。
Abstract:To explore the effect of Theragra chalcogramma skin collagen peptide on D-galactose-induced aging mice，this experiment used the prepared Theragra chalcogramma skin collagen peptide as the research object and selected low-dose（200 mg/kg），medium-dose（400 mg/kg），and high-dose（800 mg/kg）collagen peptide to build D -galactose -induced aging mouse models by gavage，so as to study the anti -aging effect of the skin.By measuring the content of moisture，collagen，hydroxyproline（HYP），and hyaluronic acid（HA）in mouse skin，superoxide dismutase（SOD），catalase（CAT），glutathione peroxidase（GSH-Px），and malondialdehyde（MDA）in mouse serum and skin and observing mouse skin tissue，the anti-aging effect of collagen peptide in Theragra chalcogrammaskin was evaluated.The results showed that Theragra chalcogramma skin collagen peptide could significantly increase the content of moisture，collagen，HYP，and HA in the skin of D-galactose-induced aging mice，and the effect of high -dose collagen peptide groupwas the most significant.In addition，Theragra chalcogramma skin collagen peptides also had good free radical scavenging ability，which could significantly increase the content of SOD，CAT，and GSH-Px in the serum and skin of aging mice and effectively inhibit the production of MDA.Through the observation of the morphology of mouse skin tissue by pathological section，it was found that Theragra chalcogramma skin collagen peptide could effectively repair the skin roughness，laxity，collagen fiber reduction，and arrangement disorder caused by aging.In summary，the Theragra chalcogramma skin collagen peptide can significantly improve the skin state of D-galactose-induced aging mice and effectively delay the skin aging process.
文章编号：202321006 中图分类号： 文献标志码：
基金项目:延边大学校企合作项目（482019012）
| Author Name | Affiliation |
| XIN Xuanying，XIE Tingyan，FU Xingpei，WANG Yan* | College of Agriculture，Yanbian University，Yanji 133000，Jilin，China |
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