Abstract:The effect of antimicrobial peptide composite preservative on the production of biogenic amines during the processing of Dosidicus gigas was examined.The inhibitory effects of oyster antimicrobial peptide（OAP），crab antimicrobial peptide（CAP），and hairtail antimicrobial peptide（HAP）on biogenic amines were analyzed by single factor tests.The optimal concentration ratio of the three antimicrobial peptides in the composite preservative was determined by orthogonal design.With total volatile basic nitrogen （TVB-N），total number of colonies，and content of biogenic amines as indicators，the effect of the composite preservative on the quality of processed D.gigas was evaluated.The results showed that the three antimicrobial peptides inhibited the production of biogenic amines.The optimal concentration ratio was determined as 2∶3∶1 for the composite preservative，which inhibited histamine by 74.39%.After the application of this composite preservative，the biogenic amine content of the test group was lower than that of the control group，which proved that this composite preservative could effectively inhibit the production of biogenic amines.On day 10，the group with application of the composite preservative showed the TVB-N of 17.87 mg/100 g，which was only 47.85% of that of the control group，proving that the composite preservative had a significant inhibitory effect on TVB-N in the samples.The composite preservative inhibited 33.02% of the total number of colonies on day 10.The results demonstrated that antimicrobial peptide composite preservative inhibited the production of biogenic amines and facilitated the quality control during the processing of D.gigas.