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食品研究与开发:2023,44(20):25-29
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水蜜桃多酚的抗氧化、抗菌和抗肿瘤活性评价
(山西农业大学 山西功能食品研究院,山西 太原 030031)
Evaluation of Antioxidant,Antibacterial and Anti-tumor Activities of Polyphenols from Peach
(Shanxi Institute for Functional Food,Shanxi Agricultural University,Taiyuan 030031,Shanxi,China)
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投稿时间:2022-06-21    
中文摘要: 为评价水蜜桃多酚提取物的体外抗氧化、抗菌和抗肿廇活性,使用乙醇从水蜜桃中提取多酚类物质,进行体外抗氧化试验来分析水蜜桃多酚对DPPH 自由基、超氧阴离子自由基、总还原力和ABTS+自由基的清除能力,通过试管法来评估水蜜桃多酚的抗菌活力,采用噻唑蓝比色法研究水蜜桃多酚提取物对肿瘤细胞增殖的影响。结果显示,水蜜桃多酚提取液对DPPH 自由基、ABTS+自由基、O2-自由基均有较高的清除率,在多酚提取物浓度为0.16 mg/mL时,清除率分别为(89.14±6.82)%、(75.83±6.36)%、(71.55±8.04)%,总还原力为1.36±0.13。水蜜桃多酚对金黄色葡萄球菌、啤酒酵母菌、链球菌、大肠杆菌、绿脓杆菌、匍枝根霉菌均表现出较好的抑制效果,尤其对匍枝根霉菌和大肠杆菌的抑菌活性最高。同时发现,水蜜桃多酚可在体外抑制人胃癌细胞SGC-7901 和人宫颈癌细胞HeLa 的增殖,具有一定的抗肿瘤活性。综上,水蜜桃多酚具有较好的抗氧化、抗菌活性和一定的抗肿瘤活性。
中文关键词: 水蜜桃多酚  抗氧化  抗菌  抗肿瘤  提取
Abstract:The objective of this paper was to evaluate the in vitro antioxidant,antibacterial,and antitumor activities of polyphenol extract of honey peach.To be specific,ethanol was used to extract polyphenols from honey peach and in vitro antioxidant assays were carried out to analyze DPPH·scavenging,superoxide anion(O2-·)scavenging,ABTS+·scavenging abilities,and total reducing power of the polyphenol extract.The test tube method was employed to assess the antibacterial activity of the polyphenol extract and MTT assay to evaluate the antitumor proliferation effect.The results showed the DPPH·,ABTS+·,and O2-·scavenging rates of the polyphenol extract were high.In the presence of polyphenols at 0.16 mg/mL,the scavenging rates hit(89.14±6.82)%,(75.83±6.36)%,and (71.55±8.04)%,respectively,and the total reducing power was 1.36±0.13.The polyphenols of honey peach showed satisfactory inhibitory effect on Staphylococcus aureus,Saccharomyces cerevisiae,Streptococcus,Escherichia coli,Pseudomonas aeruginosa,and Rhizopus stolonifer,especially R.stolonifer and E.coli.In addition,the polyphenols suppressed the proliferation of human gastric cancer SGC-7901 cells and human cervical cancer HeLa cells in vitro,suggesting the antitumor activity.In summary,polyphenols of honey peach have antioxidant,antibacterial,and antitumor activities.
文章编号:202320004     中图分类号:    文献标志码:
基金项目:山西省现代农业产业技术体系建设专项资金资助(2021-09)
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