Abstract:In order to explore the changes in characteristic metabolites of Gynostemma pentaphyllum Fu brick tea （GFT）and their potential function，the metabolites of GFT were analyzed by liquid chromatography-mass spectrometry（LC-MS）technology.Then the differential metabolites of GFT were identified，classified，and analyzed compared with the traditional brick tea，as well as their metabolic pathways.Finally，the lipid-lowering and blood sugar-lowering effects of GFT were evaluated through in vitro experiments.The results showed that compared with traditional Fu brick tea，GFT had 142 significant differential metabolites classified into 10 categories，including 38 lipids and lipid-like molecules，29 organic acids and derivatives，19 organic heterocyclic compounds，and 15 phenylpropanes and polyketides.Metabolic pathway enrichment analysis showed that the differential metabolites of GFT were mainly concentrated in lipid metabolism pathway，carbohydrate metabolism pathway，and amino acid metabolism pathway.The in vitro experiments demonstrated that GFT exhibited a lipid-lowering effect of 17.88% to 36.52% compared to the same concentration of simvastatin.Additionally，its blood sugar-lowering effect was 13.63% to 58.18% compared to the same concentration of acarbose.This study explored the characteristic metabolites of GFT and its potential lipid-lowering and blood sugar-lowering activities，providing a theoretical basis for the development of functional Fu brick tea products and promoting the diversified development of the Fu brick tea industry.