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投稿时间：2023-02-08
投稿时间：2023-02-08
中文摘要: 该文基于转录组（high-throughput transcriptome sequencing，RNA-seq）分析腐败希瓦氏菌（Shewanella putrefaciens）ATCC8071 在低温下基因表达的差异性。结果表明，在低温条件下，S.putrefaciens 中2 142 个基因的表达具有明显差异，其中对S.putrefaciens 低温冷适应机理起重要的作用基因大量集中于脂肪酸代谢、能量代谢相关通路中。进一步对S.putrefaciens 差异基因联合低温条件下脂质组学与蛋白组学数据进行分析，结果表明，在低温下S.putrefaciens 基因的差异在不同基因表达层面都表现出明显的一致性，表明脂肪酸代谢与能量代谢的改变对S.putrefaciens 冷适应贡献的机制。
Abstract:In this study，differences in the gene expression of Shewanella putrefaciens ATCC8071 at low temperatures were analyzed by high-throughput sequencing of the transcriptome sequencing（RNA-Seq）.The results showed that the expressions of 2 142 genes in S.putrefaciens were significantly different at low temperature.The genes that played an important role in the cold adaptation mechanism of S.putrefaciens at low temperature were heavily involved in fatty acid metabolism and energy metabolism pathways.Further，the analysis of S.putrefaciens differential genes combined with lipidomic and proteomic data at low temperatures showed that the S.putrefaciens gene differences at low temperatures were significantly consistent at different gene expression levels，indicating that adjustments to fatty acid metabolism and energy metabolism contribute to the S.putrefaciens cold adaptation mechanism.
keywords: Shewanella putrefaciens cold adaptation transcriptome fatty acid metabolism energy metabolism multi-omics analysis
文章编号：202315027 中图分类号： 文献标志码：
基金项目:上海城建职业学院教育科学研究项目（cjky202302）
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